MetaMorph Inc customized 3d algorithm

<t>Pre-synaptic</t> and motor end-plate volumes of diaphragm neuromuscular junctions (NMJs) from mice after 7 days of vehicle control or chloroquine treatment. ( A ) Representative images show <t>3D</t> reconstructions of diaphragm NMJs from the vehicle and chloroquine treatment groups. Pre-synaptic terminals (labeled with synaptophysin) and motor end-plates (labeled with a-bungarotoxin) show varying depth by grayscale intensity (scale bar on the right; µm). The superimposed overlap images represent apposition of pre- and post-synaptic structures, with the depth shown on the pseudocolor scale bar on the right (µm; red–white reflects the greatest apposition). Scale bar: 10 µm. ( B ) Pre-synaptic terminal volume decreased by 12% with chloroquine treatment (1248 ± 274 µm 3 ) compared to vehicle treatment (1411 ± 236 µm 3 ). *, p < 0.05 effect of treatment. Lines and whiskers represent the mean ± SD. ( C ) There was no change in motor end-plate volume with chloroquine treatment (1804 ± 269 µm 3 ) compared to vehicle treatment (1792 ± 259 µm 3 ).

Customized 3d Algorithm, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Pre-synaptic and motor end-plate volumes of diaphragm neuromuscular junctions (NMJs) from mice after 7 days of vehicle control or chloroquine treatment. ( A ) Representative images show 3D reconstructions of diaphragm NMJs from the vehicle and chloroquine treatment groups. Pre-synaptic terminals (labeled with synaptophysin) and motor end-plates (labeled with a-bungarotoxin) show varying depth by grayscale intensity (scale bar on the right; µm). The superimposed overlap images represent apposition of pre- and post-synaptic structures, with the depth shown on the pseudocolor scale bar on the right (µm; red–white reflects the greatest apposition). Scale bar: 10 µm. ( B ) Pre-synaptic terminal volume decreased by 12% with chloroquine treatment (1248 ± 274 µm 3 ) compared to vehicle treatment (1411 ± 236 µm 3 ). *, p < 0.05 effect of treatment. Lines and whiskers represent the mean ± SD. ( C ) There was no change in motor end-plate volume with chloroquine treatment (1804 ± 269 µm 3 ) compared to vehicle treatment (1792 ± 259 µm 3 ).

Journal: Cells

Article Title: Chloroquine Causes Aging-like Changes in Diaphragm Neuromuscular Junction Morphology in Mice

doi: 10.3390/cells14060390

Figure Lengend Snippet: Pre-synaptic and motor end-plate volumes of diaphragm neuromuscular junctions (NMJs) from mice after 7 days of vehicle control or chloroquine treatment. ( A ) Representative images show 3D reconstructions of diaphragm NMJs from the vehicle and chloroquine treatment groups. Pre-synaptic terminals (labeled with synaptophysin) and motor end-plates (labeled with a-bungarotoxin) show varying depth by grayscale intensity (scale bar on the right; µm). The superimposed overlap images represent apposition of pre- and post-synaptic structures, with the depth shown on the pseudocolor scale bar on the right (µm; red–white reflects the greatest apposition). Scale bar: 10 µm. ( B ) Pre-synaptic terminal volume decreased by 12% with chloroquine treatment (1248 ± 274 µm 3 ) compared to vehicle treatment (1411 ± 236 µm 3 ). *, p < 0.05 effect of treatment. Lines and whiskers represent the mean ± SD. ( C ) There was no change in motor end-plate volume with chloroquine treatment (1804 ± 269 µm 3 ) compared to vehicle treatment (1792 ± 259 µm 3 ).

Article Snippet: Volumes of pre- and post-synaptic structures were determined using a customized 3D algorithm in MetaMorph, utilizing manual thresholding as previously described [ ].

Techniques: Control, Labeling

Three-dimensional apposition of pre- and post-synaptic structures of mouse diaphragm NMJs after 7 days of vehicle or chloroquine treatment. The percent apposition was calculated as the percent of the pre-synaptic terminal directly opposing the motor end-plate. There was a significant 15% relative change (decrease) in percent apposition in the chloroquine-treated mice when compared to the vehicle. *, p < 0.05 effect of treatment. Lines and whiskers represent the mean ± SD. The 3D reconstructions shown represent examples of NMJs with 90, 60, and 30% apposition of pre- and post-synaptic structures. Depth is shown on the pseudocolor scale bar on the right (µm; red–white reflects the greatest apposition). Scale bar: 10 µm.

Journal: Cells

Article Title: Chloroquine Causes Aging-like Changes in Diaphragm Neuromuscular Junction Morphology in Mice

doi: 10.3390/cells14060390

Figure Lengend Snippet: Three-dimensional apposition of pre- and post-synaptic structures of mouse diaphragm NMJs after 7 days of vehicle or chloroquine treatment. The percent apposition was calculated as the percent of the pre-synaptic terminal directly opposing the motor end-plate. There was a significant 15% relative change (decrease) in percent apposition in the chloroquine-treated mice when compared to the vehicle. *, p < 0.05 effect of treatment. Lines and whiskers represent the mean ± SD. The 3D reconstructions shown represent examples of NMJs with 90, 60, and 30% apposition of pre- and post-synaptic structures. Depth is shown on the pseudocolor scale bar on the right (µm; red–white reflects the greatest apposition). Scale bar: 10 µm.

Article Snippet: Volumes of pre- and post-synaptic structures were determined using a customized 3D algorithm in MetaMorph, utilizing manual thresholding as previously described [ ].

Techniques:

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